Fig. 6.

Identification of VCL in angiogenesis. A The protein levels of VCL were determined by Western blot analysis in HUVECs from the CON, si-NC, si-VCL, VCL-NC and VCL groups, as well as quantification of protein expression levels. B A wound healing assay was performed to detect the migratory ability of HUVECs from the CON, si-NC, si-VCL, VCL-NC and VCL groups (magnification ×100). C Transwell assays were performed to detect the effect of HUVECs from the CON, si-NC, si-VCL, VCL-NC and VCL groups (magnification ×100). D An in-vitro Matrigel tube formation assay was performed to evaluate the angiogenic ability of HUVECs from the CON, si-NC, si-VCL, VCL-NC and VCL groups (magnification ×100). The number of branches and the tube length per field were analyzed. *P < 0.05, **P < 0.01 and ***P < 0.001. VCL, vinculin