FIGURE 5:

Interfering with organellar import or assembly of newly synthesized proteins activates Hsf1. Density plot of gene expression data for the indicated perturbation for Hsf1 targets (n = 42) and all other transcripts. “Protein import into endoplasmic reticulum:” ribosome profiling data after 30 min of depleting the signal recognition particle protein Srp72 by auxin-inducible degradation (Costa et al., 2018). “Protein import into mitochondria:” RNA-seq data after 4 h of overexpressing Psd1 (Weidberg and Amon, 2018). “Ribosomal protein assembly:” RNA-seq data after 15 min of Drg1 inhibition (diazaborine) treatment (Tye et al., 2019).