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. 2021 Aug 19;32(18):1614–1623. doi: 10.1091/mbc.E20-01-0089

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FIGURE 4: — Model for osmotic pressure-induced autophosphorylation involving changes in oligomerization and cavities. (A) Inactive dimer to phosphorylation-competent monomer equilibrium model based on the structure of WNK1SA (PDB file 6CN9). Subunit A is green, Subunit B is cyan, Activation Loops are red, and chloride is yellow. (B) WNK1SA cavities, displayed in magenta, for Subunits A and B as calculated in PyMOL. Cavities in WNK1SA (Cav1A, Cav2B, and Cav3B) are labeled. (C) Dimer interface. The Activation Loop encompassing the phosphorylation site at position 382 (alanine in WNK1SA) is inserted as a β-strand in the β-sheet of Subunit B. (D) Surface rendering of WNK1SA (same view as B) calculated in PyMOL highlighting the Cav1A indentation. (E) Surface rendering rotated 180° about the y-axis from D showing a channel and Cav3A.