FIGURE 5:

Cluster of charged residues in the Catalytic Loop and the Activation Loop in WNK1SA with associated buried water. (A) Cluster of charged residues in the WNK1SA active site with participating residues rendered in ball and stick and highlighted in purple oval. Buried waters underneath ion pairs are yellow. (B) Electron density on water molecules (contoured at 1σ). Same orientation as in A and centered on the water. (C) Stereogram of WNK1SA Catalytic Loop and Activation Loop (similar view as in A). (D) Stereogram of pWNK1 Catalytic Loop and Activation Loop (PDB 5W7T) viewed from the same perspective as C.