TABLE 1:
Comparison of hiPSC-CMs expressing WT or R134G troponin T.
| Parameter | WT | R134G | p |
|---|---|---|---|
| Traction force (μN) | 0.27 (–0.03/+0.03) | 0.17 (–0.03/+0.04) | 0.002 |
| Power (pW) | 0.10 (–0.02/+0.02) | 0.06 (–0.02/+0.03) | 0.018 |
| Contraction velocity (μm/s) | 0.33 (–0.04/+0.04) | 0.22 (–0.04/+0.05) | 0.006 |
| Relaxation velocity (μm/s) | 0.24 (–0.03/+0.03) | 0.18 (–0.03/+0.04) | 0.012 |
| Cell area (μm2) | 1000 (–50/+50) | 1800 (–200/+200) | 0.002 |
| Aspect ratio | 6.7 (–0.3/+0.2) | 6.1 (–0.5/+0.5) | 0.028 |
| Sarcomere length (μm) | 1.65 (–0.03/+0.04) | 1.77 (–0.05/+0.06) | 0.004 |
| Sarcomere length on hydrogel (μm) | 1.71 (–0.06/+0.06) | 1.75 (–0.06/+0.06) | 0.342 |
Average traction force, contraction power, and contraction and relaxation velocities of WT (n = 158) and R134G (n = 97) hiPSC-CMs were measured using traction force microscopy (see Figure 7). Average cell areas, aspect ratios, and sarcomere lengths were determined from immunofluorescence images of WT (n = 120) and R134G (n = 39) hiPSC-CMs on rectangular patterns on glass (see Figure 6). Sarcomere lengths were also measured for WT (n = 33) and R134G (n = 31) hiPSC-CMs on rectangular patterns on hydrogel. Reported uncertainties are 95% confidence intervals determined by bootstrapping.