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. 2021 Oct 1;32(20):ar1. doi: 10.1091/mbc.E21-01-0039

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© 2021 Hazan et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 5: — Association of Deup1 and SAS6 with other E2F family members. (A) Schematic representation of the full-length E2F4, E2F5, and E2F1 and their determined Deup1 and SAS6 binding abilities. (B) Cell lysates containing the indicated HA-tagged E2F species with or without Flag-Deup1 were subjected to Western blotting with the indicated α-Flag and α-HA antibodies before (Input) or after IP of Flag-Deup1. (C) Cell lysates overexpressing indicated HA-tagged E2F species with or without SAS6 were subjected to Western blotting with the indicated α-HA or α-SAS6 antibodies before (Input) or after IP with α-HA antibodies.