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. 2021 Oct 1;32(20):ar1. doi: 10.1091/mbc.E21-01-0039

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© 2021 Hazan et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 7: — E2F4 and E2F5 are not essential for primary cilia formation. (A) E2f4^f/f;E2f5^+/+ and E2f4^f/f;E2f5^–/– MEFs were transduced with lentivirus expressing Cre recombinase to ablate E2F4 and then stained via immunofluorescence for primary cilia (Arl13b, green signal) and nuclei (DAPI, blue signal). Inset is 2× main figure. Scale bar represents 15 μm. (B) Quantification shows that the percentage of cells carrying primary cilia is not significantly reduced in the double mutant MEFs relative to the E2f4^f/f;E2f5^+/+ controls. (C) Immunohistochemistry for E2F4 (brown signal) shows the efficient loss of E2F4 protein in the skin of e14.5 E2f4^f/f;E2f5^–/–;Meox2-Cre double mutant mouse embryos vs. the control E2f4^f/+;E2f5^+/–;Meox2-Cre embryos. Nuclei are counterstained (blue). (D) Adjacent skin sections from control (E2f4^f/+;E2f5^+/–;Meox2-Cre) and double knockout embryos (E2f4^f/f;E2f5^–/–; Meox2-Cre) stained by immunofluorescence for primary cilia (Arl13b, green signal) and nuclei (DAPI, blue signal) show that both form primary cilia.