FIGURE 8:

cx43^lh10 fish exhibit down-regulated vegf gene expression. Fifteen 2 dpf WT and cx43^lh10 zf embryos each were pooled, total mRNA prepared and retranscribed and mRNA levels of cx43 and vegf quantitatively amplified by qRT-PCR using specific primer pairs. Expression fold changes of three independent biological replicates were quantified (cx43, blue; and vegf, green). The box in the plot indicates the average fold change, and circles represent the fold change of each replicate. Note unchanged cx43 mRNA expression in cx43^lh10 compared with WT fish, while vegf mRNA expression is reduced significantly.