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. 2021 Oct 1;32(20):ar5. doi: 10.1091/mbc.E21-04-0224

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FIGURE 3: — Oligomerization of Mff CC region. (A) Bar diagrams of two GFP-fusion constructs: top, the CC region of Mff (GFP-CC) and bottom, the L2P mutant of the same construct (GFP-L2P). (B) vAUC of GFP-CC and GFP-L2P at 250 µM. Peak sedimentation coefficients are noted on the graph. (C) SEC profiles of GFP-CC at multiple concentrations detected at 490 nm. Absorbances normalized to peak absorbance at each group, the maximum value of each curve is normalized as 100%, the same for panel D. (D) SEC profiles of GFP-L2P at three concentrations. (E) Graph of calculated Stokes radius (from SEC results) versus GFP concentration for GFP-CC and GFP-L2P.