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. 2021 Oct 1;32(20):ar14. doi: 10.1091/mbc.E20-10-0666

FIGURE 1:

FIGURE 1:

Loss of Cdc42 GAP Rga4 leads to divergent patterns of Cdc42 dynamics in daughter cells. (A) CRIB-GFP intensity in wild-type sister cells and rga4∆ sister cells. Scale bar = 5 μm. (B) Heat map of CRIB-GFP distribution as a function of cell length in wild-type (N = 61), rga4∆ bipolar (N = 45), and rga4∆ monopolar (N = 61) cells. (C) CRIB-GFP intensity (A.U.) over time in the OE (black) and NE (gray) of a representative bipolar wild-type cell measured in 1-min intervals (N = 5). (D) Growth at the old (black) and new (gray) cell ends for the wild-type cell measured in C. The black line denotes the time at which the rate of growth changes in the new cell end (NETO). (E) CRIB-GFP intensity over time in the OE (black) and NE (gray) of a representative bipolar rga4Δ cell (N = 7) measured in 1-min intervals. (F) CRIB-GFP intensity (A.U.) over time in the OE (black) and NE (gray) of a representative monopolar rga4Δ cell (N = 7) measured in 1-min intervals.