FIGURE 8:

Distribution of Cdc42 GAP Rga6 to daughter cells is different in monopolar or bipolar rga4∆ mother cells. (A) Distribution of Rga6-3YFP in interphase control, monopolar rga4∆ and bipolar rga4∆ cells. (B) Localization of Rga6-3YFP in rga4∆ dividing cells. Scale bar = 5 μm. (C) Ratio of Rga6-3YFP intensities at the cell membrane, in the OE containing compartment over the NE containing compartment, in septated cells; in bipolar wild-type (N = 25), rga4∆ bipolar (N = 25), and rga4∆ monopolar (N = 31) cells. Statistical analysis was done using ANOVA followed by Tukey HSD post hoc test based on three independent experiments (p = 0.000). (C) Distribution of Rga6 in interphase control, monopolar rga4∆, and bipolar rga4∆ cells.