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. 2021 Oct 1;32(20):ar12. doi: 10.1091/mbc.E21-06-0303

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© 2021 Saito et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 3: — Immunostaining localization of CAMSAP3 in tracheal epithelium. (A, B) Trachea derived from P176 mice were fixed with 2% PFA and processed for coimmunostaining for CAMSAP3 and EZRIN (A) or α-tubulin (B). Images were recorded by conventional IF microscopy. IF signals were scanned along the line (not shown) drawn between the two arrows, across the cilia–BB complex from its apical (a) to basal (b) side. A typical image of three to five sections is shown for each set of immunostaining. Scale bars, 10 μm. (C) Western blots for CAMSAP3 in the trachea isolated from P172 wild-type or Camsap3^dc/dc mice.