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. 2021 Oct 1;32(20):ar8. doi: 10.1091/mbc.E21-04-0171

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© 2021 van Haastert et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 3: — Reconstitution of sGC with purified proteins. A lysate from wild-type AX3 cells was reconstituted with the indicated purified protein for 60 s on ice and then assayed for guanylyl cyclase activity at room temperature. The activity is presented relative to the control without purified protein or GTPγS added. The data shown are the means and SEM of 12 (control) or four (all others) independent reconstitutions with triplicate time points. Significance: different from control at *, P < 0.05 or ***, P < 0.001; ns, not significant at P > 0.05.