FIGURE 2.

NR4A1 dificiency aggravates canonical NLRP3 inflammasome activation and assembly in macrophages. Canonical NLRP3 inflammasome activation, BMDMs were primed for 4 h with LPS (200 ng/ml) and then stimulated with Nigericin (10 μM) for 40 min. (A‐C) Supernatants were analyzed for LDL, IL‐1β and IL‐18 release. (D and E) Immunoblot analysis of IL‐1β p17 and caspase‐1 p20 in supernatants, and immunoblot analysis of NLRP3, pro‐caspase‐1 and pro‐IL‐1β in cell lysates. (F) IP and immunoblot analysis of the interaction of endogenous NLRP3 and ASC. Values are expressed as mean ± SD, *p < 0.05, three independent experiments