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. 2021 Dec 19;11(12):e628. doi: 10.1002/ctm2.628

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© 2021 The Authors. Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Supplementation of NR improves mitochondrial functions in HFD oocytes. (A) Whole transcriptome analysis in single oocyte from Control, HFD and HFD with supplementation of NR. Heatmap of mRNA expression profiles in oocytes from Control and HFD mice, as well as HFD mice with supplementation of NR. (B) Volcano plot showing differentially expressed genes in HFD oocytes compared with Control oocytes, as well as differentially expressed genes in HFD+NR oocytes compared with HFD oocytes. Blue: downregulated; red: upregulated. (C) KEGG enrichment analysis of upregulated and downregulated expressed genes between HFD oocytes and Control oocytes. (D) KEGG enrichment analysis of upregulated and downregulated expressed genes between HFD+NR oocytes and HFD oocytes. (E) Gene ontology (GO) analysis of upregulated and downregulated expressed genes between HFD oocytes and Control oocytes. (F) GO analysis of upregulated and downregulated expressed genes between HFD+NR oocytes and HFD oocytes. (G) Mitochondrial membrane potential was assessed in oocytes from Control, HFD and HFD mice with NR supplementation by JC‐1 staining. Green fluorescence shows inactive mitochondria, while red fluorescence shows active mitochondria in oocytes. The mitochondrial membrane potential was calculated by the ratio of red to green in each oocyte. Bar = 100 μm. (H) Histogram showing JC‐1 red‐to‐green fluorescence in oocytes from each group, which reflects mitochondrial activity (n = 23 oocytes for Control, n = 21 oocytes for HFD, n = 19 oocytes for HFD + NR). (I) Transcript levels of mitochondrial fusion (mfn1, mfn2 and opa1) and fission (Drp1 and Fis1) genes in ovary detected by real‐time RT‐PCR (n = 79 oocytes for Control, n = 84 oocytes for HFD and n = 47 oocytes for HFD + NR). (J) ATP levels in oocytes from Control, HFD and HFD mice with NR supplementation (n = 30 oocytes for ND, n = 40 oocytes for HFD and n = 53 oocytes for HFD + NR). (K) Transcription of genes related to OXPHOS in oocytes from each group (n = 79 oocytes for Control, n = 84 oocytes for HFD and n = 47 oocytes for HFD + NR). ^** p < .01, ^*** p < .001