FIG. 4.

Interaction of the Nb652 mutant with Mre11 and Rad50 by immunoprecipitation and Western blot analysis. Total cell lysates were prepared from a normal control cell line (MRC5) and NBS-ILB1 cells separately infected with the pLXIN vector alone (LXIN), with the NBS1 retrovirus (NBS1), or with the Nb652 retrovirus (Nb652). Lysates were immunoprecipitated (IP) with control rabbit IgG (−) or polyclonal nibrin antiserum (α Nibrin; +). After Western transfer, the blot was probed with the antinibrin antiserum, an Mre11 monoclonal antibody, and a monoclonal anti-Rad50 antibody. The migration of molecular weight markers is indicated on the left (in kilodaltons).