Skip to main content
. 2001 Mar;21(6):2184–2191. doi: 10.1128/MCB.21.6.2184-2191.2001

FIG. 5.

FIG. 5

Subcellular localization of nibrin and Mre11 detected by immunofluorescence. Cells plated on coverslips were either untreated or exposed to 12 Gy of ionizing radiation. After 8 h, the cells were fixed and stained with antinibrin antiserum (red) and anti-Mre11 monoclonal (green). Merged panels represent overlapped images of nibrin and Mre11 staining results. (A) Localization of nibrin and Mre11 in unirradiated NBS-ILB1 cells expressing vector alone (LXIN), full-length nibrin (NBS1), nibrin fragment 5 (NbFR5), or the truncated 79-kDa form (Nb652). (B) Localization of nibrin and Mre11 in NBS-ILB1 cells expressing full-length nibrin (NBS1), nibrin fragment 5 (NbFR5), or the truncated 79-kDa form (Nb652) after irradiation. Cells were counterstained with the nuclear dye TOTO-3 (blue).