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. 2021 Dec 20;7:395. doi: 10.1038/s41420-021-00792-7

Fig. 1. Mitochondrial Gln metabolism regulates DNA damage-induced cell death.

Fig. 1

a Cell viability of immortalized MEFs treated with DOX in the presence of BPTES or DMKG (n = 3). Statistical differences were determined using a two-way ANOVA. Data represent the mean ± SD. b Survival of cells treated with ETS in the presence of BPTES or DMKG (n = 3). Cell death was measured via propidium iodide exclusion assay. c Relative caspase3/7 activity of cells treated with ETS in the presence of BPTES or DMKG (n = 4). d Cleaved caspase 3 expression of cells treated with ETS in the presence of BPTES or DMKG. β-actin serves as a loading control. e Relative viability of cells expressing a control shRNA or two independent shRNAs to GLS1 incubated with or without DMKG upon DOX treatment (n = 3). Levels of GLS1 in cells expressing a control shRNA or shRNAs to GLS1 (right). All error bars ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001.