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. 2021 Dec 6;12:778534. doi: 10.3389/fphar.2021.778534

FIGURE 1.

FIGURE 1

Purification and identification of Gr4b. (A) The spider G. Grammostola rosea. (B) RP-HPLC profile of crude venom from spider Grammostola rosea. The asterisk indicated the peak containing Gr4b. (C) Gr4b was purified to homogeneity by analytical RP-HPLC. (D) MALDI-TOF MS spectrum showing a monoisotopic M+H+ of 4348.6 Da. (E) Sequence alignment of Gr4b with similar toxins in NaSpTx family2; black lines show the disulfide linkage.