TABLE 4.
The effects of 250 nM Gr4b on activation of Nav1.9 mutants.
| Control | 250 nM Gr4b | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Voltage dependence of Activation (mV) | Voltage dependence of Inactivation (mV) | Voltage dependence of Activation (mV) | Voltage dependence of Inactivation (mV) | |||||||||
| V1/2 | k | n | V1/2 | k | n | V1/2 | k | n | V1/2 | k | n | |
| Nav1.9 N1139A | −51.5 ± 3.8 | 7.2 ± 1.0 | 5 | −63.9 ± 3.0 | 9.5 ± 0.5 | 6 | −43.8 ± 4.5** | 12.9 ± 2.1* | 5 | −60.6 ± 2.8 | 11.9 ± 0.6* | 6 |
| Nav1.9 N1139K | −51.1 ± 2.3 | 8.8 ± 0.9 | 8 | −68.5 ± 3.9 | 9.6 ± 0.5 | 4 | −52.1 ± 1.8 | 8.8 ± 0.7 | 8 | −49.7 ± 13.0 | 19.6 ± 2.7* | 4 |
| Nav1.9 L1140A | −52.1 ± 0.9 | 6.3 ± 0.2 | 5 | −71.3 ± 2.8 | 10.5 ± 0.9 | 7 | −42.6 ± 1.6** | 13.2 ± 1.2** | 5 | −45.0 ± 7.1** | 15.6 ± 0.8** | 7 |
| Nav1.9M1141A | −54.7 ± 2.7 | 7.2 ± 0.5 | 5 | −76.3 ± 2.7 | 9.9 ± 0.2 | 5 | −55.5 ± 2.8 | 10.3 ± 2.0 | 5 | −37.6 ± 7.1** | 21.9 ± 1.0*** | 5 |
| Nav1.9 L1143A | −53.1 ± 1.8 | 6.7 ± 0.4 | 6 | −68.3 ± 3.4 | 10.9 ± 0.3 | 5 | −58.7 ± 1.8* | 8.1 ± 5.0** | 6 | −63.5 ± 2.2* | 10.3 ± 0.6 | 5 |
| Nav1.9 L1143V | −51.9 ± 2.2 | 7.2 ± 0.2 | 6 | −79.9 ± 4.8 | 11.8 ± 0.4 | 4 | −50.8 ± 3.9 | 11.4 ± 1.3* | 6 | −44.3 ± 3.8** | 14.9 ± 1.8 | 4 |
| Nav1.9 S1145A | −49.1 ± 2.6 | 7.4 ± 0.4 | 7 | −63.9 ± 2.5 | 8.8 ± 0.4 | 8 | −37.8 ± 4.1** | 15.0 ± 1.6** | 7 | −17.0 ± 5.0**** | 20.1 ± 2.1** | 8 |
Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, when compared with Control. Parametric paired two-tailed t-test was used. n is presented as the number of the separate experimental cells.