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Analysis of MAPK and Smad2 signaling pathways in LβT2 cells treated with CB NPs. LβT2 cells were cultured for the indicated times with 100 μg/mL of CB NPs. Total and phosphorylated Erk1/2, p38, JNK and Smad2 were analyzed by immunoblotting as indicated in Materials and Methods. Seven independent experiments were performed and a representative immunoblot is shown for each protein.
