FIGURE 8.

Effects of CB NPs on GnRH agonist stimulation of Fshb and Lhb expression in LβT2 cells. LβT2 cells were cultured for 24 h with increasing concentrations (25–100 μg/mL) of CB NPs, washed and incubated or not for an additional 6 h with the GnRH agonist Triptorelin [GnRHa, 100 nM; (A)] or Activin A [10 ng/mL; (B)]. Total RNAs were extracted, and Fshb and Lhb mRNA levels were determined by real-time quantitative PCR as indicated in Materials and Methods. Data are expressed as percentage over GnRHa (A) or Activin A (B) stimulation in absence of CB NPs and are the mean ± SEM from 3 to 6 independent experiments. Statistical differences were determined by non-parametric Kruskal-Wallis test followed by Dunnet’s multiple comparison test. *p < 0.05 compared to no CB NPs.