FIGURE 1.

A Western blot analysis of human ACE2s, produced in Nicotiana benthamiana plants. dACE2: angiotensin-converting enzyme 2 (ACE2) co-expressed with bacterial Endo H, produced in N. benthamiana, different concentration (dilutions) of crude extract were loaded into wells; gACE2: western blot analysis of human ACE2, produced in N. benthamiana plants; different concentration (dilutions) of crude extract were loaded into wells; C-undiluted crude extract from non-infiltrated N. benthamiana, was loaded into well; gPA83: 25, 50, and 100 ng of purified plant produced dPA83 of Bacillus anthracis, loaded as a control protein to quantify the expression levels of ACE2 and ACE2 proteins. Purified anti-His Tag antibody (Cat. No. 652502, BioLegend) was used as primary and mouse IgG used as secondary antibodies to detect ACE2 proteins.