FIGURE 6.

The metabolic state of erythrocytes governs the dependency of GSSG efflux on intracellular GSSG content. Amount of cellular GSSG efflux in relation to cellular GSSG content upon DHA treatment in the absence and presence of glucose or 2-DG, respectively. Values were calculated from flow cytometry GSH depletion data (Figure 3) and as described in the section “Results.” Data of GSSG efflux upon DHA treatment (± glucose or 2-DG, respectively) are derived from Figure 4. Graphs combine these data to highlight the correlation of GSSG efflux with cellular GSSG content under various conditions. (A) Dependency of GSSG efflux on cellular GSSG levels upon pre-treatment of cells with various amounts of DHA (data from Figures 3C, 4B). (B) Dependency of GSSG efflux on cellular GSSG levels upon pre-treatment of cells with 2 mM DHA and various amounts of glucose (black) or 2-DG (gray) (data from Figures 3D, 4D). (C) Overlay of DHA (black) data from panel (A) with 2-DG (gray) data from panel (B).