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. 2021 Dec 6;12:758785. doi: 10.3389/fpls.2021.758785

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Copyright © 2021 Jia, Liu, Geng and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibitory effects of ACS2/6 activation and ACC treatment on SDD1 expression and protein accumulation. (A) RT-qPCR-based relative levels of SDD1 mRNA transcripts in immature leaves of WT, acs2-1, acs6-1, acs2-1acs6-1, ACS2-OE(#1), ACS2-OE(#3), ACS6-OE(#1), and ACS6-OE(#3) seedlings. The experiment was repeated three times with consistent results. Values are means ± SD (Student’s t-test; *P < 0.05; ^**P < 0.01). (B) Evaluation of SDD1 protein levels by western blotting. The fusion protein GFP-SDD1 was collected from immature leaves of pSDD1::SDD1-GFP-expressing WT or acs2-1acs6-1 lines with or without drought treatment. Levels of GFP-SDD1 fusion protein were determined using GFP antibody. GAPDH protein was used as a loading control. The experiment was repeated three times with consistent results.