Skip to main content
. 2021 Dec 10;118(50):e2107993118. doi: 10.1073/pnas.2107993118

Fig. 6.

Fig. 6.

p62 targets Cys/N-degron substrates to autophagy via UBA domain. (A) ICC analysis of SH-SY5Y cells under p62 interference and treated with BAF (200 nM, 6 h), tBHP (250 μΜ, 6 h) or both. (Scale bar, 10 μm.) (B) WB with same conditions as A. (C) Co-IP assay of HEK293T cells expressing C-RGS4-FLAG with full-length or ΔUBA p62MYC and treated with BAF, Tbhp, or both (same as A). (D) Same as C but with RD-CDC6FLAG. (E) Same as A but expressing p62 WT and ΔUBA. (F) Chemical structure of the Nt-Arg mimic ZZ-ligand YOK-1104.