Fig. 1.

Tilt series collection and the missing wedge issue. (Left) Schematic of the tilt series collection scheme. Sample projections are acquired over a range of tilt angles, typically from –60${}^{°}$ to +60${}^{°}$. (Right) The middle slice of the kxkz plane shows the missing wedge (MW) and baby missing wedges (BMWs) of information visualized in Fourier space. Attenuation of high frequencies due to radiation damage as the tilt series progresses is not depicted (15, 16).