. 2021 Dec 10;118(50):e2112986118. doi: 10.1073/pnas.2112986118

Table 2.

Binding affinities of 3PG for the hPGK wild type and various mutants in the absence and presence of MgADP and their kinetic parameters^*

	K_D/mM		α	K_M/mM	k_cat/s⁻¹
	−MgADP	+MgADP	α	K_M/mM	k_cat/s⁻¹
WT	0.112 (0.106 to 0.119)	0.251 (0.239 to 0.263)	0.448 (0.426 to 0.470)	0.20 ± 0.06	795 ± 50
K215A	0.108 (0.102 to 0.113)	0.150 (0.146 to 0.155)	0.716 (0.695 to 0.739)	nd^†	nd^†
D374K	0.110 (0.104 to 0.115)	0.140 (0.132 to 0.147)	0.785 (0.745 to 0.829)	nd^†	nd^†
D374N	0.111 (0.106 to 0.115)	0.0656 (0.0656 to 0.0675)	1.69 (1.64 to 1.69)	0.16 ± 0.03	315 ± 15

*The K_D values in the presence of MgADP are calculated from the α-values (see Fig. 1C). Values in brackets represent the 95% bootstrap CIs. K_M and k_cat values, with their SDs, are calculated from three independent experiments.

†Not detectable.