Fig. 3.

The LC1 domain of hnRNPH1 is required for interactions with different kinds of RNA regulatory proteins. (A) Different regions of hnRNPH1 in the form of GFP-linked protein at equal molar concentrations were incubated with mCherry hydrogel droplets composed of LC domains of FUS, TAF15, DHX9, hnRNPA1, hnRNPA2, and hnRNPF. The intensity of the hydrogel binding was analyzed using confocal microscopy. (B) HEK-293T cells were transfected with Flag-tagged WT, ΔLC1, or ΔLC2 hnRNPH1 constructs. Immunoprecipitation was performed using anti-Flag antibodies, and coprecipitated proteins were analyzed by Western blotting using antibodies for indicated proteins. Representative images are shown from more than three independent immunoprecipitation experiments.