Fig. 1.

Endocytic proteins with PLDs form thermosensitive puncta. (A) Reversible, temperature-dependent dissolution and formation of Sla1-labeled endocytic puncta when cells are cycled above and below an apparent T_t of 45.3 °C (yellow region) over multiple heating and cooling cycles (Left; mean ± SD; n > 8 cells). (B) Representative images of temperature-dependent dissolution and reformation of Sla1-labeled endocytic puncta in GPD1Δ cells treated with Lat A when cells are cycled above and below a T_t over a heating and cooling cycle. (Scale bar, 4 μm.) (C) Average number of puncta per cell is bistable as a function of temperature.