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Experimental timeline from human pluripotent stem cell seeding, their directed cardiac differentiation, and hvCAS fabrication to an action potential and calcium transient data acquisition. The photos are of human embryonic stem cells (hES2), which show the cells’ morphology at different time points of directed cardiac differentiation. The photos are taken at 40X magnification. Cardiomyocyte yield from hES2 shows 89.5% cTnT+ cells, which were then used for downstream experiments.
