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. 2021 Dec 6;9:681665. doi: 10.3389/fcell.2021.681665

FIGURE 4.

FIGURE 4

Human embryonic stem cell (hES2)-derived human ventricular cardiac anisotropic sheet (hvCAS) paced at 1.5 Hz during acute hypokalaemic (hypoK) experiment where the treatment was for 15 min before data acquisition. Number of samples is represented by n where the first number refers to control (5 mM KCl in Tyrode solution) samples and the second number refers to hypoK (3 mM KCl in Tyrode solution) samples. (A) Action Potential (AP) Time-to-peak. (n = 14, 17). (B) Calcium Transient (CaT) Time-to-peak. (n = 12, 11). (C) AP Upstroke Velocity measured by the change in fluorescence intensity per millisecond. ΔF represents the change in fluorescence intensity. (n = 9, 10). (D) CaT Upstroke Velocity measured by the change in fluorescence intensity per millisecond. ΔF represents the change in fluorescence intensity. (n = 11, 10) Time-to-peak of AP and CaT increased in hypokalaemic condition (asterisks, p < 0.001, p < 0.05 respectively). Similarly Upstroke Velocity of AP and CaT increased in hypokalaemic condition (asterisks, p < 0.05, p < 0.001 respectively).