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. 2021 Dec 6;9:788461. doi: 10.3389/fbioe.2021.788461

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Copyright © 2021 Shu, Wang, Zhang, Li, Le and Chang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In situ-formed anti-inflammatory hydrogel loaded with plasmid DNA encoding VEGF for healing of burn wounds. (A) Application of this hydrogel in a model of a splinted excisional wound (schematic). (B) Immunohistochemical staining showing expression of the proinflammatory cytokines IL-1β and TNF-α. (C) Immunohistochemical staining showing expressions of the angiogenic factors VEGF, CD31, and α-SMA. Scale bar = 100 μm. (D) RT-qPCR of mRNA expression for inflammatory and angiogenic factors (IL-1β, TNF-α, VEGF). **p ≤ 0.01, *p ≤ 0.05. Reproduced with permission from (Wang et al., 2019).