Fig. 1.

Real-time imaging of Aβ aggregation and endothelial cell death. (A) hBMECs co-incubated with 20 μM Aβ₄₂ and 30 nM QDAβ were observed by an inverted fluorescence microscope. Time series of images shows the gradual steps of Aβ₄₂ aggregation and cell death. The yellow dotted line indicates the outline of an endothelial cell. (B) Left panel; 3D images of an hBMEC stained with F-actin with Alexa488 phalloidin. Right panel; 3D images of Aβ aggregates co-incubated under the same conditions as in (A). Images were captured by a confocal microscope. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)