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MDP5 inhibited NF-κB downstream cytokine production in BMDMs. BMDMs were pretreated with MDP5 (10–100 ug/ml) for 6 h then stimulated with Pam3CSK4 for cytokine production. Mock was shown by using BMDMs without Pam3CSK4. IL-6 (A) and TNF-α (B) production and viability (C) of BMDMs was measured after 12 h of Pam3CSK4-induced stimulation. Data are the mean ± S.D. (n = 5). **p < 0.01, ***p < 0.001. Data were analyzed using the Student’s t-test (two-tailed).
