Table 6.

Effects of dietary Ecobiol supplementation on cytokines level of broilers¹.

Item2	Ecobiol (mg/kg)					SEM	P value
	0	500	1,000	1,500	2,000		Linear	Quadratic
D 21
Serum (pg/mL)
IL-1β	31.45	30.65	29.75	28.44	30.22	0.86	0.094	0.150
IL-6	61.92	53.73	52.17	51.59	52.96	2.08	0.004	0.014
TNF-α	108.60	108.85	92.32	96.17	100.29	3.83	0.021	0.058
IL-10	31.96	36.99	41.82	38.69	41.13	1.89	0.002	0.072
D 42
Serum (pg/mL)
IL-1β	33.18	30.45	27.35	27.47	28.79	1.23	0.005	0.019
IL-6	62.91	61.84	61.89	55.15	60.98	1.80	0.072	0.306
TNF-α	111.38	104.50	104.66	89.93	99.38	4.02	0.005	0.245
IL-10	29.60	34.15	34.71	35.71	33.44	1.54	0.067	0.028
Jejunum (pg/mg)
IL-10	21.37	24.89	24.70	20.24	23.24	1.67	0.864	0.403
IL-1β	32.74	23.17	25.03	23.43	26.24	1.22	0.002	<0.001
IL-6	129.73	107.02	93.69	104.31	109.88	5.04	0.012	<0.001
TNF-α	144.30	128.30	131.73	117.44	132.79	5.23	0.048	0.028
Ileum (pg/mg)
IL-10	22.86	21.07	24.20	25.49	21.73	1.55	0.662	0.327
IL-1β	42.92	31.68	25.43	28.98	28.88	1.93	<0.001	<0.001
IL-6	154.22	131.38	118.78	104.93	118.32	5.87	<0.001	0.003
TNF-α	162.63	146.53	133.52	127.31	115.68	6.37	<0.001	0.514

¹Data are the means of 8 replicates per treatment.

²Abbreviations: IL-1β, interleukin-1β; IL-6, interleukin-6; TNF-α, tumor necrosis factor-α; IL-10, interleukin-10.