Table 2.

Astrocyte optimal seeding densities

Culture plate	Volume of media per well	Number of cells
12 well	1.5 mL	4–5 × 105
24 well	1 mL	2–2.5 × 105
48 well	0.5 mL	105
96 well Seahorse	0.1–0.2 mL	30,000–40,000

Continue to step 34 for astrocyte silencing or directly to step 40 for astrocyte stimulation.