Table 3.
Culture plate | Opti-MEM volume | INTERFERin volume | Amount of siRNA | Volume of DMEM per well |
---|---|---|---|---|
24 well | 100 μL | 2 μL | 0.6 pmoles | 500 μL |
48 well | 75 μL | 1 μL | 0.3 pmoles | 300 μL |
96 well Seahorse | 50 μL | 0.5 μL | 0.17 pmoles | 100 μL |
Culture plate | Opti-MEM volume | INTERFERin volume | Amount of siRNA | Volume of DMEM per well |
---|---|---|---|---|
24 well | 100 μL | 2 μL | 0.6 pmoles | 500 μL |
48 well | 75 μL | 1 μL | 0.3 pmoles | 300 μL |
96 well Seahorse | 50 μL | 0.5 μL | 0.17 pmoles | 100 μL |