Reagent | Final concentration | Amount |
---|---|---|
DMEM F12 | 1× | 475 mL |
N1 Medium Supplement 100× | 1× | 5 mL |
BSA Fraction V 7.5% | 0.075% | 5 mL |
P/S 10,000 U/mL | 100 U/mL | 5 mL |
HEPES 1M | 100 mM | 5 mL |
NEAA 100× | 1× | 5 mL |
Total | n/a | 500 mL |
Reagent | Final concentration | Amount |
---|---|---|
DMEM F12 | 1× | 475 mL |
N1 Medium Supplement 100× | 1× | 5 mL |
BSA Fraction V 7.5% | 0.075% | 5 mL |
P/S 10,000 U/mL | 100 U/mL | 5 mL |
HEPES 1M | 100 mM | 5 mL |
NEAA 100× | 1× | 5 mL |
Total | n/a | 500 mL |