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. 2021 Dec 15;3(1):101033. doi: 10.1016/j.xpro.2021.101033
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins

Poly-L-lysine Sigma-Aldrich # P4707
DPBS no calcium no magnesium Gibco # 14190144
HBSS no calcium no magnesium Gibco # 14170112
75 cm2/250mL Flasks Falcon # 353136
0.25% Trypsin-EDTA Gibco # 25200072
70 micron Nylon Cell Strainers Fisherbrand # 22-363-548
DMEM F12 with Glutamax Gibco #10565042
DMEM no glucose no glutamine Gibco #A1443001
OptiMEM Gibco #31985070
Fetal Bovine Serum - heat inactivated (FBS-HI) Gibco #10-438-026
Penicillin-Streptomycin (10,000 U/mL) (P/S) Gibco #15140122
HEPES 1M Gibco #15630080
N1 Medium Supplement Sigma-Aldrich #N6530
Glucose Solution 200 g/L Gibco #A2494001
MEM Non-Essential Amino Acids Solution (NEAA) Gibco #11140050
Bovine Albumin Fraction V (7.5% solution) Gibco #15260037
INTERFERin Polyplus Transfection #409
Buffer RLT Qiagen #79216
recombinant mouse IFNg R&D Systems #485-MI-100
recombinant mouse TNFa R&D Systems #410-MT-010
recombinant mouse IL1b R&D Systems #401-ML-005
recombinant mouse IFNb R&D Systems #8234-MB-010/CF
recombinant human TGFb Miltenyi Biotec #130-095-067
recombinant human TGFa R&D Systems #239-A-100
recombinant mouse VEGF-B R&D Systems #751-VE-025
recombinant mouse IL-17 R&D Systems #421-ML-025/CF
Complement Component C1q Native Protein MyBioSource #MBS143105
recombinant mouse IL-10 R&D Systems #417-ML-005
recombinant GM-CSF PeproTech #315-03
XF DMEM medium, pH 7.4 Agilent Technologies 103575-100
XF 1.0 M Glucose solution Agilent Technologies 103577-100
XF 100 mM Pyruvate solution Agilent Technologies 103578-100
XF 200 mM Glutamine solution Agilent Technologies #103579-100

Oligonucleotides

SMARTpool siRNAs Dharmacon N/A
ON-TARGETplus Non-targeting Pool Dharmacon D-001810-10

Critical commercial assays

Seahorse XF Cell Mito Stress Test Kit Agilent Technologies #103015-100
CyQUANT Cell Proliferation Assay Kit, for cells in culture Thermo Fisher Scientific #C7026

Experimental Models: Organisms/Strains

Mouse: C57BL/6J 1–3 days old mouse pups The Jackson Laboratory #000664

Other

Conical 50 and 15 mL tubes Falcon #352196 #352070
70 μm cell strainers Corning #431751
Serological pipets Greiner Bio-One #606180 #607180
Tissue Culture Plates Corning #3596 #3526
Centrifuge N/A N/A
Incubator N/A N/A
Shaker with controlled temperature VWR #76407-108
XFe96 Seahorse analyzer Agilent Technologies N/A