Chemicals, peptides, and recombinant proteins |
|
Poly-L-lysine |
Sigma-Aldrich |
# P4707 |
DPBS no calcium no magnesium |
Gibco |
# 14190144 |
HBSS no calcium no magnesium |
Gibco |
# 14170112 |
75 cm2/250mL Flasks |
Falcon |
# 353136 |
0.25% Trypsin-EDTA |
Gibco |
# 25200072 |
70 micron Nylon Cell Strainers |
Fisherbrand |
# 22-363-548 |
DMEM F12 with Glutamax |
Gibco |
#10565042 |
DMEM no glucose no glutamine |
Gibco |
#A1443001 |
OptiMEM |
Gibco |
#31985070 |
Fetal Bovine Serum - heat inactivated (FBS-HI) |
Gibco |
#10-438-026 |
Penicillin-Streptomycin (10,000 U/mL) (P/S) |
Gibco |
#15140122 |
HEPES 1M |
Gibco |
#15630080 |
N1 Medium Supplement |
Sigma-Aldrich |
#N6530 |
Glucose Solution 200 g/L |
Gibco |
#A2494001 |
MEM Non-Essential Amino Acids Solution (NEAA) |
Gibco |
#11140050 |
Bovine Albumin Fraction V (7.5% solution) |
Gibco |
#15260037 |
INTERFERin |
Polyplus Transfection |
#409 |
Buffer RLT |
Qiagen |
#79216 |
recombinant mouse IFNg |
R&D Systems |
#485-MI-100 |
recombinant mouse TNFa |
R&D Systems |
#410-MT-010 |
recombinant mouse IL1b |
R&D Systems |
#401-ML-005 |
recombinant mouse IFNb |
R&D Systems |
#8234-MB-010/CF |
recombinant human TGFb |
Miltenyi Biotec |
#130-095-067 |
recombinant human TGFa |
R&D Systems |
#239-A-100 |
recombinant mouse VEGF-B |
R&D Systems |
#751-VE-025 |
recombinant mouse IL-17 |
R&D Systems |
#421-ML-025/CF |
Complement Component C1q Native Protein |
MyBioSource |
#MBS143105 |
recombinant mouse IL-10 |
R&D Systems |
#417-ML-005 |
recombinant GM-CSF |
PeproTech |
#315-03 |
XF DMEM medium, pH 7.4 |
Agilent Technologies |
103575-100 |
XF 1.0 M Glucose solution |
Agilent Technologies |
103577-100 |
XF 100 mM Pyruvate solution |
Agilent Technologies |
103578-100 |
XF 200 mM Glutamine solution |
Agilent Technologies |
#103579-100 |
|
Oligonucleotides |
|
SMARTpool siRNAs |
Dharmacon |
N/A |
ON-TARGETplus Non-targeting Pool |
Dharmacon |
D-001810-10 |
|
Critical commercial assays |
|
Seahorse XF Cell Mito Stress Test Kit |
Agilent Technologies |
#103015-100 |
CyQUANT Cell Proliferation Assay Kit, for cells in culture |
Thermo Fisher Scientific |
#C7026 |
|
Experimental Models: Organisms/Strains |
|
Mouse: C57BL/6J 1–3 days old mouse pups |
The Jackson Laboratory |
#000664 |
|
Other |
|
Conical 50 and 15 mL tubes |
Falcon |
#352196 #352070 |
70 μm cell strainers |
Corning |
#431751 |
Serological pipets |
Greiner Bio-One |
#606180 #607180 |
Tissue Culture Plates |
Corning |
#3596 #3526 |
Centrifuge |
N/A |
N/A |
Incubator |
N/A |
N/A |
Shaker with controlled temperature |
VWR |
#76407-108 |
XFe96 Seahorse analyzer |
Agilent Technologies |
N/A |