Yeast lysis buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| EDTA (0.5 M) | 5 mM | 0.2 mL |
| proteinase inhibitor buffer(100×) | 1× | 0.2 mL |
| PBS | n/a | 19.6 mL |
| Total | n/a | 20 mL |
[prepare before using]
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