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. 2021 Dec 1;12:752782. doi: 10.3389/fimmu.2021.752782

Figure 3.

Figure 3

B-cell subset phenotyping in PBMC. (A) Absolute counts of B-cell subsets in PBMC of HIV+ (n = 39), 6-8 weeks post-ART (n = 29) and HC (n = 19) participants. (B) Opt-SNE projections of expression of the indicated markers on CD19+ B cells pooled from a subset of participants, pre-ART (n = 11), post-ART (n = 11), and HC (n = 11). (C) Opt-SNE projection of pooled CD19+ B-cell clusters identified by FlowSOM clustering. Fifteen clusters from FlowSOM analysis were visualized as a heatmap of mean fluorescence intensity, each row as a different cluster, while columns represent analyzed markers, and frequency of B cells from each group in FlowSOM clusters indicated. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by bootstrapped Welch Two Sample t-test with 10,000 iterations on full set; data are not statistically significant unless noted.