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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Exp Hematol. 2020 Nov 7;93:70–84.e4. doi: 10.1016/j.exphem.2020.11.002

Figure 1. Inhibition of the TGFβ pathway promotes clonogenic growth of HSPCs from FA mice.

Figure 1.

A) Western blots of the lysates from murine bone marrow cells. Lin− cells from bone marrow of wild-type mice were cultured for 2 h in presence of TGFβ1 (5 ng/ml), TGFβ2 (5 ng/ml) or TGFβ3 (5 ng/ml) with or without AVID200. Note that two types of antibodies were used to detect the phospho-SMAD2 levels. One of the antibodies was used only against phospho-Smad2 (p-Smad2) whereas the other one was against both phosphor-Smad2 and Smad3 (p-Smad2/3). A representative blot is shown.

B) TGFβ pathway inhibitors promote colony formation of murine Fancd2−/− HSPCs. Lin− cells from wild-type (WT) or Fancd2−/− mice were cultured for 7 days in methylcellulose medium with inhibitors of the TGFβ pathway, namely, SD208 (10 μM), 1D11 (10 μg/mL) and AVID200 (0.2 ng/mL). Hematopoietic colonies were quantified (n=3).

p-values of 0.01 to 0.05 were considered significant (*), p-values of 0.001 to 0.01 were considered very significant (**) and p-values of <0.001 were considered extremely significant (****). Data in (B) are represented as mean ± SEM. See also Supplementary Figure 1.