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. 2021 Dec 20;19:437. doi: 10.1186/s12951-021-01138-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Characterization of hESC-Exosomes. a TEM images of 2D-Exo and 3D-Exo. Original magnification, 40,000×, Scale bar, 100 nm. b Western blot analysis of CD9 and CD63 in 2D-Exo and 3D-Exo. c Size distribution of 2D-Exo and 3D-Exo analyzed by NTA in scatter mode. d CLSM images of 2D-Exo and 3D-Exo labeled with PKH26. Red: PKH26-Exo. Blue: DAPI. Original magnification, 40×, Scale bar, 50 μm