Fig. 6.

3D-Exo suppressed TGFβ-induced LX2 cell activation by delivering miR-6766-3p. a According to microarray analysis, 11 miRNAs were selected to verify their expressions by qPCR in 2D-Exo and 3D-Exo. b miR-6766-3p was highly expressed in 3D-hESCs, compared with 2D-hESCs. c Venn diagrams showing the intersection between predicted target genes of miR-6766-3p. d Go terms enriched functions for target genes of miR-6766-3p. e KEGG enriched pathways for target genes of miR-6766-3p. f A schematic drawing showing the putative binding sites of wt-TGFβRII and mut-TGFβRII in miR-6766-3p, and that the 3ʹ untranslated regions (UTRs) of TGFβRII including wild-type (WT) or mutant type (MUT) were respectively cloned downstream of the Renilla luciferase (Rluc) gene as fusion gene driven by SV40 promoter. g miR-6766-3p mimics or NC was co-transfected with a dual-luciferase (Rluc and the firefly luciferase (fluc)) reporter construct containing TGFβRII WT or TGFβRII MUT into HEK-293 T cells. Relative luciferase reporter activity were shown by Rluc activity versus fluc activity (Rluc/fluc). Data represent the mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001