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PSF-A interacts with Sin3A in vitro. Different domains of Sin3A were expressed as GST-fusion proteins in E. coli. Equivalent amounts of these fusion proteins were incubated with in vitro-synthesized 35S-PSF-A. The glutathione-agarose beads were washed and the bound 35S-PSF-A analyzed by SDS-gel electrophoresis followed by autoradiography. PSF-A binds to the region of Sin3A (amino acids 404 to 545) between the PAH2 and PAH3 domains.
