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The repressor activity of PSF-A requires amino acids 361 to 464 of the protein. PSF-A deletion mutants, created by PCR, spanning various regions of the N-terminal domain of PSF-A (amino acids 1 to 464) were cloned as Gal4 fusion constructs. The region mediating the repressor activity of PSF-A was functionally mapped by transfection studies using the G5pBLCat2 reporter in HeLa cells.
