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. 2001 Apr;21(7):2312–2323. doi: 10.1128/MCB.21.7.2312-2323.2001

FIG. 4.

FIG. 4

Polypeptide composition and developmental expression pattern of dMediator. (A) Immunoprecipitation of dMediator with anti-dSOH1 beads. Proteins immunoprecipitated from buffer IP100 (lane 1) or the Mono S dMediator peak fraction (lane 2) were electrophoresed on SDS–11.5% polyacrylamide gels and silver stained. Identified dMediator components are indicated at the right. Immunoglobulin heavy (IgH) and light (IgL) chains are indicated at the left. (B) Immunoblot analysis of dMediator proteins immunoprecipitated with nonspecific anti-β-galactosidase (NS), anti-dSOH1 (S), and anti-Trfp (T). The input Mono S fraction (I) is shown for comparison. (C) Superose-6 chromatography of the dMediator complex. The Mono S peak fractions (I) were fractionated on a Superose-6 column, and the fractions (numbers above the lanes) were subjected to SDS-PAGE and analyzed by immunoblotting with the Abs indicated at the left. The elution positions of size markers (in kilodaltons) are indicated by arrows. The size markers used were blue dextran (2,000 kDa; void volume), thyroglobulin (667 kDa), ferritin (440 kDa), and bovine serum albumin (66 kDa). (D) Developmental expression pattern of dMediator homologs. Poly(A) RNA (4 μg per lane) from Drosophila embryos (E), first (L 1)-, second (L 2)-, and third (L 3)-instar larvae, white prepupae (PP), day 1 (P D1), day 2 (P D2), and day 3 (P D3) pupae, and adults (A) were electrophoresed on a formaldehyde agarose gel and probed with radiolabled DNA fragments derived from the dMediator genes indicated at the left. rp49 was used as a positive control.

HHS Vulnerability Disclosure