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. 2021 Dec 20;12:607. doi: 10.1186/s13287-021-02672-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Induction of E12.5_GSCLCs by small molecules. A Schematic illustration of in vitro chemical induction strategy. B qPCR detection of Gata4 and Foxl2 expression in GSCLCs induced by treatment with different small-molecule compounds. Bars = Mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001. C qPCR detection of Gata4 and Foxl2 expression in GSCLCs induced by AM580 and V580. Bars = Mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001. D Immunofluorescence staining of Gata4 (green) and Foxl2 (red) in GSCLCs induced by V580. Scale bar = 20 μm. E Protein levels of pluripotency markers (Nanog and Oct4) and E12.5_GSCs markers (Gata4 and Foxl2) in GSCLCs induced by V580 were determined via western blot analysis. β-actin served as a loading control. F Heatmap highlighting DEGs compared with E12.5_GSCs, determined using RNA-seq. G PCA shows that V580_D6 GSCLCs and E12.5_GSCs were closer with regard to the overall transcriptome