Fig. 4.

V580_D6 GSCLCs, but not MEF and P_6w, promote meiosis. A Morphology of different cell types after aggregating with PGCs. Scale bar = 50 μm. B The diameter of aggregates. The size of MEF_aggregates was obviously smaller, and P_6w_aggregates did not form round structures even with PHA treatment, whereas Gonad_aggregates and V580_D6 aggregates did not change considerably in size. Bars = Mean ± SEM (n = 10). *P < 0.05; **P < 0.01; ***P < 0.001. C E12.5 female gonad reaggregation supports normal meiosis initiation in PGCs; V580_D6 GSCLCs can also support the normal meiosis of PGCs. In aggregates of MEF or P_6w with PGCs, only a small amount of Sycp3 expression was observed, and the expression of Sycp1 was inhibited or completely suppressed in some cases, resulting in the inability of PGCs to proceed with normal meiosis. Scale bar = 20 μm. D Proportion of PGCs that can initiate meiosis in the different groups. Sycp3⁺ cells could not be detected in MEF_ and P_6w_ aggregates. Bars = Mean ± SEM (n = 10). *P < 0.05; **P < 0.01; ***P < 0.001. E Percentage of normal synaptonemal complexes in different groups based on pachytene spread (n = 40). Bars = Mean ± SEM (n = 10). *P < 0.05; **P < 0.01; ***P < 0.001. F Statistics of Mlh1 foci per cell at the pachytene stage. No Mlh1 foci formed in MEF_ and P_6w_ aggregates. Bars = Mean ± SEM (n ≥ 10). *P < 0.05; **P < 0.01; ***P < 0.001. Scale bar = 20 μm